human non small cell lung cancer cell line a549  (ATCC)

Journal: Journal of Translational Medicine

Article Title: Developing an antibody drug encapsulation nanoagent targeting HER2 for cancer treatment

doi: 10.1186/s12967-025-07450-x

Figure Lengend Snippet: Effects of Tr-ACT2 on a lung cancer cell model. ( A ) Effects of Tr-ACT2 on the growth of A549 cells at concentrations of 0.05 µM, 0.25 µM, and 1 µM for indicated time. Cell growth was evaluated using the MTT assay. ( B ) Representative images of A549 cell morphology at 48 h after treatment with 0.25 μM Tr-ACT2 or vehicle. Scale bar: 400 μm. ( C , D ) Western blot analysis showing the protein levels of PARP, cleaved PARP, IgG heavy chain, IgG light chain, and GAPDH in A549 cells treated with 0.25 μM Tr-ACT2 for indicated time. Protein levels are quantified by densitometry and normalized by GAPDH, versus 0 h. Data are presented as means ± SD ( n ≥ 4). p > 0.05 (ns), p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****)

Article Snippet: Human breast cancer cell lines SKBR3, JIMT1, BT549, MDA-MB-231, and the human non-small cell lung cancer (NSCLC) cell line A549 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), and cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% Fetal bovine serum (FBS) and 1% Antibiotic-Antimycotic, at 37 °C and 5% CO2.

Techniques: MTT Assay, Western Blot

Journal: Journal of Translational Medicine

Article Title: Developing an antibody drug encapsulation nanoagent targeting HER2 for cancer treatment

doi: 10.1186/s12967-025-07450-x

Figure Lengend Snippet: Antitumor efficacy of Tr-ACT2 in the A549 xenograft model. ( A ) Tumor volume change over time for all mice. ( B ) Normalized body weight change over time for all mice. Data are presented as means ± SEM ( n ≥ 3)

Article Snippet: Human breast cancer cell lines SKBR3, JIMT1, BT549, MDA-MB-231, and the human non-small cell lung cancer (NSCLC) cell line A549 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), and cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% Fetal bovine serum (FBS) and 1% Antibiotic-Antimycotic, at 37 °C and 5% CO2.

Techniques:

Journal: Frontiers in Immunology

Article Title: Study on the spatiotemporal regulation of interferon-stimulated genes during Zika virus infection

doi: 10.3389/fimmu.2025.1702266

Figure Lengend Snippet: Fold change in the expression of the 11 interferon-stimulated genes (ISGs) in A549 cells after Zika virus (ZIKV) infection. Fold change in the expression of the 11 genes in A549 cells was determined 24 h after mock infection or ZIKV infection [multiplicity of infection (MOI) = 1] using RT-PCR. Gene expression was normalized to GAPDH and calculated using the 2 −ΔΔ C t method. Data are presented as the mean ± SD from at least three independent experiments. Statistical significance was assessed using a two-sided **p < 0.01; ***p < 0.001;****p < 0.0001.

Article Snippet: The human non-small cell lung cancer cell line A549 (purchased from the American Type Culture Collection, catalog no. CCL-185) was used.

Techniques: Expressing, Virus, Infection, Reverse Transcription Polymerase Chain Reaction, Gene Expression

Journal: Frontiers in Immunology

Article Title: Study on the spatiotemporal regulation of interferon-stimulated genes during Zika virus infection

doi: 10.3389/fimmu.2025.1702266

Figure Lengend Snippet: Effects of seven interferon-stimulated gene (ISG) knockdown on Zika virus (ZIKV) replication in A549 cells. A549 cells were transfected with small interfering RNA (siRNA) targeting candidate ISGs or non-targeting control siRNA for 48 h, followed by infection with ZIKV at a multiplicity of infection (MOI) of 1. The viral copy number in the supernatant was measured by quantitative real-time PCR (qRT-PCR) at 24 h post-infection. Data represent the mean ± SD from three or more independent experiments. Significance was determined using two-sided Student’s t -test. * p < 0.05; ** p < 0.005.

Article Snippet: The human non-small cell lung cancer cell line A549 (purchased from the American Type Culture Collection, catalog no. CCL-185) was used.

Techniques: Knockdown, Virus, Transfection, Small Interfering RNA, Control, Infection, Real-time Polymerase Chain Reaction, Quantitative RT-PCR