non small cell lung cancer cell lines pc 9  (ATCC)

Journal: Biochemistry and Biophysics Reports

Article Title: ATM inhibition restores IFN-γ sensitivity and induces ferroptosis in NSCLC via DNA damage response

doi: 10.1016/j.bbrep.2026.102568

Figure Lengend Snippet: IFN-γ responsiveness correlates DNA damage and repair responses in NSCLC cell lines (A) Relative cell viability of A549 or PC-9 treated with the indicated concentration of IFN-γ for 24 h are shown. Data are presented as mean ± SD. (B) Reactome analysis of the GSE180942 dataset was performed using differential CRISPR β-scores under IFN-γ treatment (Δβ = PC-9 − A549). Bars indicate the normalized enrichment score (NES) for each pathway indicated. Positive NES denotes pathways whose constituent genes are more essential in A549, whereas negative NES denotes pathways more essential in PC-9 upon IFN-γ treatment.

Article Snippet: The human non-small cell lung cancer cell lines PC-9 (kindly gifted from Dr. Kiura, Okayama University, Japan) and A549 (CCL-185, obtained from American Type Culture Collection) were used.

Techniques: Concentration Assay, CRISPR

Journal: Biochemistry and Biophysics Reports

Article Title: ATM inhibition restores IFN-γ sensitivity and induces ferroptosis in NSCLC via DNA damage response

doi: 10.1016/j.bbrep.2026.102568

Figure Lengend Snippet: Inhibition of ATM restores NSCLC cells to IFN-γ by inducing DNA damage response (A) Cell viability of A549 (left panel) or PC-9 (right panel) treated with IFN-γ (1000 ng/ml) and/or KU-55933 (10 μM) for 24 h are shown. Data are presented as mean ± SD. * p < 0.05. (B) Expression of γH2AX and b-Actin (loading control) in A549 (left panel) or PC-9 (right panel) cells treated with IFN-γ (1000 ng/ml) and/or KU-55933 (10 μM) for 24 h are shown.

Article Snippet: The human non-small cell lung cancer cell lines PC-9 (kindly gifted from Dr. Kiura, Okayama University, Japan) and A549 (CCL-185, obtained from American Type Culture Collection) were used.

Techniques: Inhibition, Expressing, Control

Journal: Biochemistry and Biophysics Reports

Article Title: ATM inhibition restores IFN-γ sensitivity and induces ferroptosis in NSCLC via DNA damage response

doi: 10.1016/j.bbrep.2026.102568

Figure Lengend Snippet: Inhibition of ATM in combination with IFN-γ induce ferroptosis in NSCLCs Cell viability of A549 (A) or PC-9 (B) treated with the indicated combination of IFN-γ (1000 ng/ml), KU-55933 (10 μM), Ferrostatin-1 (5 μM), and Liproxstatin-1 (5 μM) for 24 h are shown. Data are presented as mean ± SD. * p < 0.05.

Article Snippet: The human non-small cell lung cancer cell lines PC-9 (kindly gifted from Dr. Kiura, Okayama University, Japan) and A549 (CCL-185, obtained from American Type Culture Collection) were used.

Techniques: Inhibition

Journal: Pharmaceutical Biology

Article Title: Mechanistic insight into the anti-inflammatory and lung-protective effects of Agrimonia pilosa extract via NF-κB/MAPK inhibition in ovalbumin- and lipopolysaccharide-induced respiratory inflammation models

doi: 10.1080/13880209.2026.2627661

Figure Lengend Snippet: Effects of AP on the mRNA expression of inflammatory cytokines and mediators in LPS-stimulated A549 cells. A549 cells were pretreated with AP for 1 h, followed by LPS stimulation for 24 h. The mRNA expression levels of (A) IL-1β, (B) IL-6, (C) TNF-α, (D) iNOS, (E) COX-2, and (F) MUC5AC were analyzed by qRT-PCR. The data are presented as mean ± SEM ( n = 3). # p < 0.05, ### p < 0.001 vs. normal control; * p < 0.05, *** p < 0.001 vs. LPS treated control.

Article Snippet: Cells from the human non-small cell lung cancer (NSCLC) cell line A549 were purchased from Korean Cell Line Bank (KCLB No. 10185, Seoul, Korea) and cultured in RPMI-1640 medium (10–040, Corning Inc., New York, NY, USA) supplemented with 10% fetal bovine serum (Corning Inc.) and 1% penicillin-streptomycin (Cat. No.50-195-9713, GenDEPOT Inc., Baker, TX, USA).

Techniques: Expressing, Quantitative RT-PCR, Control

Journal: Pharmaceutical Biology

Article Title: Mechanistic insight into the anti-inflammatory and lung-protective effects of Agrimonia pilosa extract via NF-κB/MAPK inhibition in ovalbumin- and lipopolysaccharide-induced respiratory inflammation models

doi: 10.1080/13880209.2026.2627661

Figure Lengend Snippet: Effects of AP on the NF-κB and MAPK signaling pathway in A549 cells. Cells were pretreated with AP for 1 h, followed by LPS stimulation for 6 h. Protein expression levels were analyzed by Western blots. Phosphorylation levels of NF-κB, p38, ERK, and JNK were evaluated to assess activation of the NF-κB and MAPK signaling pathways. β-actin was used as a loading control. Densitometric analysis of the phosphorylated proteins was normalized to total protein levels and is presented as bar graphs. The data are presented as ### p < 0.001 vs. normal control; *** p < 0.001 vs. LPS treated control.

Article Snippet: Cells from the human non-small cell lung cancer (NSCLC) cell line A549 were purchased from Korean Cell Line Bank (KCLB No. 10185, Seoul, Korea) and cultured in RPMI-1640 medium (10–040, Corning Inc., New York, NY, USA) supplemented with 10% fetal bovine serum (Corning Inc.) and 1% penicillin-streptomycin (Cat. No.50-195-9713, GenDEPOT Inc., Baker, TX, USA).

Techniques: Expressing, Western Blot, Phospho-proteomics, Activation Assay, Protein-Protein interactions, Control